Rasilo M L, Wartiovaara J, Renkonen O
Can J Biochem. 1980 May;58(5):384-93. doi: 10.1139/o80-050.
Human teratocarcinoma derived cells, line PA 1, maintained in the undifferentiated state, yielded upon exhaustive pronase digestion unusually large glycopeptides (fraction A), which showed on gel filtration an apparent molecular weight larger than 7400. These glycopeptides derived from whole cell proteins carried large-sized oligosaccharides as evidenced by repeated pronase treatments, hydrazinolysis, and beta-elimination experiments. The oligosaccharides consisted of mannose, fucose, galactose, and N-acetylglucosamine. The PA 1 cells contained also oligomannosyl type glycans, presumably linked to asparagine (fraction C glycopeptides). These glycopeptides were strongly bound to Con A--Sepharose and their oligosaccharides were released by endo-beta-N-acetylglucosaminidase H. The liberated glycans ranged from Man5GlcNAc to Man9GlcNAc as analyzed by paper chromatography. "Pulse-chase" experiments suggest that there is a precursor-product relationship between the mannose label in the fraction C (oligomannosyl type) glycopeptides and the fraction A glycopeptides.
维持在未分化状态的人畸胎瘤衍生细胞系PA 1，经彻底的链霉蛋白酶消化后产生了异常大的糖肽（组分A），这些糖肽在凝胶过滤中显示出明显大于7400的分子量。通过重复的链霉蛋白酶处理、肼解和β-消除实验证明，这些源自全细胞蛋白质的糖肽带有大尺寸的寡糖。这些寡糖由甘露糖、岩藻糖、半乳糖和N-乙酰葡糖胺组成。PA 1细胞还含有低聚甘露糖型聚糖，可能与天冬酰胺相连（组分C糖肽）。这些糖肽与伴刀豆球蛋白A-琼脂糖紧密结合，它们的寡糖可被内切β-N-乙酰葡糖胺糖苷酶H释放。通过纸色谱分析，释放出的聚糖范围从Man5GlcNAc到Man9GlcNAc。“脉冲追踪”实验表明，组分C（低聚甘露糖型）糖肽中的甘露糖标记与组分A糖肽之间存在前体-产物关系。
