Broughton W J, van Egeraat A W, Lie T A
Can J Microbiol. 1980 Apr;26(4):562-5. doi: 10.1139/m80-099.
Nodulation of Pisum sativum cv. Afghanistan by Rhizobium leguminosarum strain Tom can be blocked by the nonnodulating R. leguminosarum strain PF2. This system was used to study the dynamics of rhizobial binding to seedling root surfaces. Appreciable numbers of 35S-labeled rhizobia bound to the root surface within 2h after inoculation. PF2 colonized the root surfaces earlier and in greater numbers that did Tom. Radioactive extracellular polysaccharides and lipopolysaccharides from either organism failed to bind to the seedling roots in measurable quantities.
豌豆品种阿富汗被豆科根瘤菌菌株汤姆结瘤的过程可被非结瘤的豆科根瘤菌菌株PF2阻断。该系统用于研究根瘤菌与幼苗根表面结合的动态过程。接种后2小时内，可观数量的35S标记根瘤菌结合到根表面。PF2比汤姆更早且以更大数量定殖于根表面。来自任一菌株的放射性胞外多糖和脂多糖均未能以可测量的量结合到幼苗根上。
