Gomulkievich Ia, Popdimitrova N, Kunchev N, Zlatarev G
Eksp Med Morfol. 1980;19(2):92-7.
Membrane permeability for different ions may successfully be evaluated under conditions of ionic equilibrium, which provides osmotic equilibrium in the cell and steadiness of its volume. The present investigation was promoted on red cells of experimental animals under conditions of ionic equilibrium, using P32 with up to 30 microCi activity. Unidirectional penetration of ions in the red cells was studied. The speed constant K0 was derived from the graphic relation InA=f(t) as an angular coefficient at the starting moment. The type of ions was shown to influence K0 value, which was maximal in the presence of tartarate ions in the suspension medium and minimal in the presence of succinate ions. This study demonstrated that the activation energy does not depend on the ions in the solution. The results are highly suggestive of the existence of differences in the mechanism of penetration of different ions through the red cell membrane.
在离子平衡条件下可成功评估不同离子的膜通透性，离子平衡能确保细胞内的渗透平衡及其体积的稳定。本研究是在离子平衡条件下，使用活性高达30微居里的P32对实验动物的红细胞进行的。研究了离子在红细胞中的单向渗透。速度常数K0是从InA=f(t)的图形关系中得出的，作为起始时刻的角系数。结果表明离子类型会影响K0值，在悬浮介质中存在酒石酸盐离子时K0值最大，而在存在琥珀酸盐离子时最小。本研究表明活化能不依赖于溶液中的离子。这些结果强烈暗示不同离子透过红细胞膜的渗透机制存在差异。
