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Active site structural change of alpha-chymotrypsin due to 2-halogeno-ethanols; comparison with ethanol, I-propanol, and urea.

作者信息

Ebina S, Nagai Y

出版信息

Experientia. 1980 May 15;36(5):534-5. doi: 10.1007/BF01965782.

DOI:10.1007/BF01965782
PMID:7379943
Abstract

2-Halogeno-ethanols change the active site structure of alpha-chymotrypsin more rapidly and effectively than ethanol, 1-propanol and urea, probably before producing an extensive conformation change.

摘要

相似文献

1
Active site structural change of alpha-chymotrypsin due to 2-halogeno-ethanols; comparison with ethanol, I-propanol, and urea.
Experientia. 1980 May 15;36(5):534-5. doi: 10.1007/BF01965782.
2
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Denaturing agents (urea, acrylamide) protect enzymes against irreversible thermoinactivation: a study with native and immobilized alpha-chymotrypsin and trypsin.变性剂(尿素、丙烯酰胺)可保护酶免受热不可逆失活:天然及固定化α-胰凝乳蛋白酶和胰蛋白酶的研究
FEBS Lett. 1975 Mar 1;51(1):152-5. doi: 10.1016/0014-5793(75)80874-x.

本文引用的文献

1
MODIFICATION OF A METHIONINE RESIDUE NEAR THE ACTIVE SITE OF CHYMOTRYPSIN BY P-NITROPHENYL BROMOACETYL-ALPHA-AMINOISOBUTYRATE.对硝基苯基溴乙酰-α-氨基异丁酸对胰凝乳蛋白酶活性位点附近甲硫氨酸残基的修饰
Biochemistry. 1965 Mar;4:377-86. doi: 10.1021/bi00879a001.
2
Alkylation of chymotrypsin by alpha-bromo-4-nitroacetophenone, a charge-transfer acceptor.用α-溴-4-硝基苯乙酮(一种电荷转移受体)对胰凝乳蛋白酶进行烷基化反应。
J Am Chem Soc. 1967 Mar 29;89(7):1747-8. doi: 10.1021/ja00983a046.
3
Unfolding reactions of proteins. II. Spectral and optical rotatory dispersion studies in urea, guanidinium chloride, and 2-chloroethanol.
蛋白质的去折叠反应。II. 在尿素、氯化胍和2-氯乙醇中的光谱和旋光色散研究。
Biochemistry. 1967 Jun;6(6):1638-50. doi: 10.1021/bi00858a010.
4
Unfolding reactions of proteins. I. Kinetic and equilibrium measurements of diisopropylphosphorylchymotrypsin and chymotrypsinogen in urea.
Biochemistry. 1966 Apr;5(4):1230-41. doi: 10.1021/bi00868a017.
5
Inhibition of alpha-chymotrypsin by 2-halogeno-ethanols; comparisons with 2-methyl-ethanols and urea.2-卤代乙醇对α-糜蛋白酶的抑制作用;与2-甲基乙醇和尿素的比较。
Experientia. 1979 Aug 15;35(8):1011-2. doi: 10.1007/BF01949911.