Morioka K, Asami Y, Tanaka K, Ono T, Saheki S, Harada-Saheki K, Tanaka T
Gan. 1980 Feb;71(1):146-50.
The isozyme patterns of glycolytic enzymes of Friend leukemia cells (FLC) were compared with those of erythrocytes and erythroblasts. Erythrocyte-specific R types of pyruvate kinase (PK) were clearly observed in phenylhydrazine-induced mouse erythroblast, and much less amount of them was also observed in Friend leukemia cells. When FLC were induced to differentiate by hexamethylene-bisacetamide (HMBA), the R types were slightly reduced. When the induction of differentiation was inhibited by 12-O-tetradecanoylphorbol 13-acetate (TPA), the R types and M2-R hybrids rather increased. These results are reverse of those obtained when hemoglobin production is used as a marker of differentiation. Isozyme patterns of lactic dehydrogenase and aldolase did not change during differentiation of FLC induced by HMBA, and were the same as those of mouse erythroblasts and erythrocytes.
将弗瑞德白血病细胞（FLC）糖酵解酶的同工酶模式与红细胞和成红细胞的同工酶模式进行了比较。在苯肼诱导的小鼠成红细胞中清晰观察到了红细胞特异性的丙酮酸激酶（PK）R型，在弗瑞德白血病细胞中也观察到了少量该类型。当用六亚甲基双乙酰胺（HMBA）诱导FLC分化时，R型略有减少。当用12 - O - 十四酰佛波醇13 - 乙酸酯（TPA）抑制分化诱导时，R型和M2 - R杂种反而增加。这些结果与以血红蛋白产生作为分化标志物时所获得的结果相反。在HMBA诱导的FLC分化过程中，乳酸脱氢酶和醛缩酶的同工酶模式没有变化，与小鼠成红细胞和红细胞的同工酶模式相同。
