Domke I, Ross W, Weis W
Hoppe Seylers Z Physiol Chem. 1980 Apr;361(4):591-3.
The microsomal NADH- as well as the ascorbate-dependent fatty acid desaturation are diminished by monodehydroascorbate. This effect can be neutralized by borate. Borate forms an adduct with didehydroascorbate and thus prevents the formation of monodehydroascorbate. The ascorbate-dependent oleate formation is increased significantly by the same reaction (p less than 0.01). The NADH-dependent reaction is diminished by parallel reactions of the desaturase system and L-ascorbate: ferricytochrome b5 oxidoreductase at ferrocytochrome b5. In the case of the ascorbate-dependent reaction the diminished oleate formation is caused by the more positive oxidation state of cytochrome b5 in presence of monodehydroascorbate.
微粒体NADH以及抗坏血酸依赖性脂肪酸去饱和作用会被单脱氢抗坏血酸减弱。这种效应可被硼酸盐中和。硼酸盐与二脱氢抗坏血酸形成加合物，从而阻止单脱氢抗坏血酸的形成。相同反应会使抗坏血酸依赖性油酸形成显著增加（p小于0.01）。NADH依赖性反应会因去饱和酶系统与L-抗坏血酸：亚铁细胞色素b5氧化还原酶在亚铁细胞色素b5处的平行反应而减弱。在抗坏血酸依赖性反应中，油酸形成减少是由于在单脱氢抗坏血酸存在下细胞色素b5的氧化态更正。
