Scherer G, Weis W
Hoppe Seylers Z Physiol Chem. 1978 Nov;359(11):1527-30. doi: 10.1515/bchm2.1978.359.2.1527.
The microsomal enzyme ascorbate-cytochrome b5 reductase participates in the ascorbate-dependent fatty acid desaturation. Three pieces of evidence are given for this statement: 1) Comparison of the rate of ascorbate-dependent oleate formation with the rate of reduction of cytochrome b5 in microsomes and in the isolated detergent form shows that only the enzymatic reduction of cytochrome b5 is fast enough to support oleate formation; 2) added enriched ascorbate-cytochrome b5 reductase increases the rate of return of stearoyl-CoA oxidised cytochrome b5 back to the reduced state; 3) addition of enriched ascorbate-cytochrome b5 reductase increases the rate of ascorbate-dependent oleate formation in rat liver microsomes.
微粒体酶抗坏血酸 - 细胞色素b5还原酶参与依赖抗坏血酸的脂肪酸去饱和作用。关于这一说法有三条证据：1）将微粒体中以及分离出的去污剂形式下依赖抗坏血酸的油酸形成速率与细胞色素b5的还原速率进行比较，结果表明只有细胞色素b5的酶促还原速率足够快，能够支持油酸的形成；2）添加富集的抗坏血酸 - 细胞色素b5还原酶可提高被硬脂酰辅酶A氧化的细胞色素b5恢复到还原状态的速率；3）添加富集的抗坏血酸 - 细胞色素b5还原酶可提高大鼠肝脏微粒体中依赖抗坏血酸的油酸形成速率。
