Okubo S, Morimoto M, Mineura K, Marumo H, Omura S
J Antibiot (Tokyo). 1980 Feb;33(2):231-5. doi: 10.7164/antibiotics.33.231.
The effect of prumycin on the mouse immune system was studied. Prumycin at a dose of 75 mg/kg (1/2LD50) showed essentially no suppression of hemolytic plaque forming cells (PFC) against sheep red blood cells (SRBC) when it was administered on day 0, 1 or 2. However, a weak suppression was observed by administration of prumycin on day 3. Also a remarkable suppression of PFC was demonstrated when mouse spleen cells were pre-incubated with prumycin at the concentration over 62.5 mg/kg in vitro. The delayed-type hypersensitivity reaction against SRBC was remarkably suppressed by cyclophosphamide, but it was not much by prumycin, except by administration on the day of elicitation. Prumycin of a high concentration inhibited an uptake of 3H-thymidine by mouse spleen lymphocytes incubated with or without phytohemagglutinin, but that of a low concentration, 1.9 approximately 0.03 mcg/ml, slightly increased the blastogenic stimulation of lymphocytes.
研究了普鲁霉素对小鼠免疫系统的影响。当在第0、1或2天给药时，剂量为75mg/kg（1/2LD50）的普鲁霉素对针对绵羊红细胞（SRBC）的溶血空斑形成细胞（PFC）基本没有抑制作用。然而，在第3天给药时观察到了微弱的抑制作用。此外，当小鼠脾细胞在体外与浓度超过62.5mg/kg的普鲁霉素预孵育时，PFC出现了显著抑制。环磷酰胺可显著抑制针对SRBC的迟发型超敏反应，但除了在激发当天给药外，普鲁霉素对其抑制作用不大。高浓度的普鲁霉素可抑制与或未与植物血凝素一起孵育的小鼠脾淋巴细胞对3H-胸腺嘧啶核苷的摄取，但低浓度（1.9至约0.03mcg/ml）的普鲁霉素可轻微增强淋巴细胞的增殖刺激作用。
