苹果中萘乙酰胺残留量的高压液相色谱测定法
High pressure liquid chromatographic determination of naphthaleneacetamide residues in apples.
作者信息
Cochrane W P, Lanouette M, Grant R
出版信息
J Assoc Off Anal Chem. 1980 Jan;63(1):145-8.
A simple, rapid, and accurate method has been developed for the determination of naphthalene-acetamide (NAAmide) residues in apples. After extraction with chloroform, separation is performed on an RP-8 high pressure liquid chromatographic column using acetonitrile-water (30 + 70) buffered to pH 3.5 at a flow rate of 1.7 mL/min. Either a variable wavelength ultraviolet detector set at 220 nm or a fluorometric detector can be used for quantitation. Average recoveries at the 0.01 and 0.1 ppm spiking levels were 83 and 89%, respectively. Incorporation into a previously described method for naphthaleneacetic acid (NAA) residues in apples has resulted in a procedure for the samultaneous determination of NAA and NAAmide.
已开发出一种简单、快速且准确的方法来测定苹果中萘乙酰胺（NAAmide）的残留量。用氯仿萃取后，在RP - 8高压液相色谱柱上进行分离，使用乙腈 - 水（30 + 70），缓冲至pH 3.5，流速为1.7 mL/分钟。可使用设置在220 nm的可变波长紫外检测器或荧光检测器进行定量。在0.01 ppm和0.1 ppm加标水平下的平均回收率分别为83%和89%。将该方法纳入先前所述的苹果中萘乙酸（NAA）残留量测定方法，已形成了同时测定NAA和NAAmide的程序。
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