丁香假单胞菌菜豆致病变种对菜豆毒素不敏感的鸟氨酸氨甲酰基转移酶：对菜豆毒素免疫的基础
Phaseolotoxin-insensitive ornithine carbamoyltransferase of Pseudomonas syringae pv. phaseolicola: basis for immunity to phaseolotoxin.
作者信息
Staskawicz B J, Panopoulos N J, Hoogenraad N J
出版信息
J Bacteriol. 1980 May;142(2):720-3. doi: 10.1128/jb.142.2.720-723.1980.
Abstract
Cell-free extracts from phaseolotoxin-producing strains of Pseudomonas syringae pv. phaseolicola grown at 18 degrees C, the optimum temperature for phaseolotoxin production, contain an ornithine carbamoyltransferase activity that is insensitive to phaseolotoxin. Extracts from the same strains grown at 30 degrees C, a temperature at which little or no detectable phaseolotoxin is produced, and from phaseolotoxin-nonproducing strains contain a phaseolotoxin-sensitive ornithine carbamoyltransferase activity. The phaseolotoxin-insensitive ornithine carbamoyltransferase activity is also less senstive to N delta-(phosphonacetyl)-L-ornithine than the phaseolotoxin-sensitive ornithine carbamoyltransferase activity of the corresponding strain.
摘要
在18摄氏度（丁香假单胞菌菜豆致病变种产生相毒素的最适温度）下生长的产相毒素丁香假单胞菌菜豆致病变种菌株的无细胞提取物，含有对相毒素不敏感的鸟氨酸氨甲酰基转移酶活性。在30摄氏度（此温度下产生的相毒素很少或无法检测到）下生长的相同菌株的提取物，以及不产相毒素菌株的提取物，含有对相毒素敏感的鸟氨酸氨甲酰基转移酶活性。与相应菌株对相毒素敏感的鸟氨酸氨甲酰基转移酶活性相比，对相毒素不敏感的鸟氨酸氨甲酰基转移酶活性对Nδ-（膦酰乙酰）-L-鸟氨酸也不太敏感。
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