Ferrier V, Jaylet A
Chromosoma. 1978 Oct 20;69(1):47-63. doi: 10.1007/BF00327380.
Eggs of diploid females of Pleurodeles waltii, fertilized artificially by waltlii sperm, have been submitted to increased temperature of hydrostatic pressure during the first hour of development. Of the resulting viable individuals about 95% were triploid, the remainder diploid or tetraploid. Other types of ploidy have been observed in abnormal embryos (n, 2n/4n, 5n, n/5n, 6n). Some of the treated eggs came from females with a marker chromosome (pericentric inversion). The karyotypes of the animals developing from such eggs confirm that triploidy results from retention of the second polar body and make it possible to interpret the origin of the other types of ploidy. From the results it can be envisaged how gynogenetic animals can be obtained from eggs inseminated by inactivated sperm and shock-treated to restore diploidy.
用华氏钝口螈精子人工授精的华氏钝口螈二倍体雌性卵子，在发育的第一小时受到了静水压力升高的处理。在产生的存活个体中，约95%是三倍体，其余为二倍体或四倍体。在异常胚胎中还观察到了其他类型的倍性（单倍体、二倍体/四倍体、五倍体、单倍体/五倍体、六倍体）。一些处理过的卵子来自带有标记染色体（臂间倒位）的雌性。由此类卵子发育而来的动物的核型证实，三倍体是由于第二极体的保留产生的，并且使得解释其他类型倍性的起源成为可能。从这些结果可以设想，如何从用灭活精子授精并经休克处理以恢复二倍性的卵子中获得雌核发育动物。
