Mitra A K, Baustian C L, Mikkelson T J
J Pharm Sci. 1980 May;69(3):257-61. doi: 10.1002/jps.2600690303.
A derivatization procedure and a high-performance liquid chromatographic (HPLC) method of analysis for pilocarpine are described. The method is based on the quaternization of the 3'-tertiary amino group of the methylimidazole ring of pilocarpine with p-nitrobenzyl bromide. The HPLC system employs and RP-ODS column with a methanol-water mobile phase containing octanesulfonate as an ion-pairing agent. The sensitivity of the method permits the analysis of pilocarpine in biological fluids such as aqueous humor. The method is selective for pilocarpine in the presence of isopilocarpine. Its applicability to the analysis of aromatic heterocyclic and alkyl tertiary amines is demonstrated.
本文描述了毛果芸香碱的衍生化方法及高效液相色谱（HPLC）分析方法。该方法基于毛果芸香碱甲基咪唑环3'-叔氨基与对硝基苄基溴的季铵化反应。HPLC系统采用RP-ODS柱，以含有辛烷磺酸盐作为离子对试剂的甲醇-水作为流动相。该方法的灵敏度可用于分析生物流体如房水中的毛果芸香碱。该方法在存在异毛果芸香碱时对毛果芸香碱具有选择性。证明了其在芳香族杂环和烷基叔胺分析中的适用性。
