一种用于测量培养淋巴细胞磷脂合成活性的快速方法。
A rapid method for measuring the phospholipid synthetic activity of incubated lymphocytes.
作者信息
Kageyama K, Kinoshita Y, Kimura E
出版信息
Experientia. 1978 Oct 15;34(10):1311-2. doi: 10.1007/BF01981438.
PMID:738403
Abstract
A rapid and simple method for estimating the phospholipid synthetic activity of incubated lymphocytes by a Florisil column technique following the uptake of 14C-oleic acid was established. Stimulation of phospholipid synthesis by PHA and inhibitions caused by Tween 20 or Tween 80 and heating were evaluated easily with this method.
摘要
建立了一种快速简便的方法，通过弗罗里硅土柱技术，在14C-油酸摄取后估计培养淋巴细胞的磷脂合成活性。利用该方法可以轻松评估PHA对磷脂合成的刺激作用以及吐温20或吐温80和加热所引起的抑制作用。
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