用于体外分流的固定化L-苯丙氨酸解氨酶多管反应器。

Multitubular reactors with immobilized L-phenylalanine ammonia-lyase for use in extracorporeal shunts.

作者信息

Kalghatgi K, Horváth C, Ambrus C M

出版信息

Res Commun Chem Pathol Pharmacol. 1980 Mar;27(3):551-61.

Abstract

Hollow fiber cartridges of improved design have been loaded with L-phenylalanine ammonia-lyase [EC 4.3.1.5.] from Rhodotorula glutinis in order to prepare multitubular enzyme reactors for removal of L-phenylalanine from blood. The kinetics of the enzyme in free solution was investigated and the results showed a behavior similar to that reported for L-phenylalanine ammonia-lyase from other sources. On the other hand kinetic data with the reactors were obtained in a recirculating system by measuring the increase in trans-cinnamate concentration in the perfusate. The effect of changing the enzyme load in the hollow fiber cartridge, the length of the cartridge, the substrate concentration, pH as well as the flow rate and the temperature have been investigated. The results showed that the overall kinetics of the enzymic reaction in the multitubular reactor is not the same as that in free solution and the difference can be attributed to diffusion resistances in the hollow fiber system.

摘要

为了制备用于从血液中去除L-苯丙氨酸的多管式酶反应器，已将改进设计的中空纤维柱装填了来自粘红酵母的L-苯丙氨酸解氨酶[EC 4.3.1.5]。研究了该酶在游离溶液中的动力学，结果表明其行为与其他来源的L-苯丙氨酸解氨酶报道的行为相似。另一方面，通过测量灌注液中反式肉桂酸浓度的增加，在循环系统中获得了反应器的动力学数据。研究了改变中空纤维柱中酶负载量、柱长度、底物浓度、pH值以及流速和温度的影响。结果表明，多管式反应器中酶促反应的整体动力学与游离溶液中的不同，这种差异可归因于中空纤维系统中的扩散阻力。