Khaw B A, Fallon F T, Strauss H W, Haber E
Science. 1980 Jul 11;209(4453):295-7. doi: 10.1126/science.7384803.
Antibodies, by virtue of marked selectivity and affinity, may lend themselves to identification of structures of unique antigenic specificity in vivo. In experimental myocardial infarction in dogs, F(ab')2 fragments of antibodies to cardiac myosin that had been labeled with iodine-131 were shown to localize within the lesion. Because the energy characteristics of iodine isotopes are not ideal for imaging with a gamma camera, a new method for labeling antibody fragments with divalent or polyvalent radionuclides was developed. A bifunctional chelating agent, diethylenetriamine pentaacetic acid was covalently coupled, by an amide bond, to Fab fragments of antibodies to canine cardiac myosin. A stable chelate was then formed with indium-111, a nuclide that has appropriate half-life and energy characteristics for gamma imaging. Antibodies treated in this way retain their antigen-binding activity and are useful in locating myocardial infarcts in vivo.
抗体凭借其显著的选择性和亲和力，可用于在体内识别具有独特抗原特异性的结构。在犬实验性心肌梗死中，用碘-131标记的抗心肌肌球蛋白抗体的F(ab')2片段显示定位于病变部位。由于碘同位素的能量特性对于γ相机成像并不理想，因此开发了一种用二价或多价放射性核素标记抗体片段的新方法。一种双功能螯合剂，二乙烯三胺五乙酸通过酰胺键与抗犬心肌肌球蛋白抗体的Fab片段共价偶联。然后与铟-111形成稳定的螯合物，铟-111是一种具有适合γ成像的半衰期和能量特性的核素。以这种方式处理的抗体保留其抗原结合活性，可用于在体内定位心肌梗死。
