用尿素处理的人宫颈中胶原蛋白结构的超微结构变化。
Ultrastructural changes in the architecture of collagen in the human cervix treated with urea.
作者信息
Kischer C W, Droegemueller W, Shetlar M, Chvapil M, Vining J
出版信息
Am J Pathol. 1980 Jun;99(3):525-38.
Injection of a solution of 30% urea in acetate buffer at pH 4.0 into the stroma of the uterine cervix results in a marked change in the mechanical properties of the tissue with subsequent easy dilatation. This study analyzes the changes in the collagenous matrix of the cervix by ultrastructural and biochemical methods. Collagen fibrils in urea-treated sections of the cervix are swollen and unravelled, showing spiral configuration of subunits in both cross-sections and longitudinal sections. The regular localization of ruthenium-red-positive material is absent in urea-treated tissues. Chemical analysis of incubated cervical tissues shows a reduction of the total glycosaminoglycans and of dermatan sulfate with release of the latter into the medium. It is suggested that urea dissociates intercollagen linkages by solubilizing a certain glycosaminoglycan, possibly dermatan sulfate. After this solubilization the collagen fibril is prompted to unwind, resolving the collagen microfibrils, which appear to be organized in a spiral fashion.
向子宫颈基质中注射pH 4.0的30%尿素醋酸盐缓冲溶液会导致组织力学性能发生显著变化，随后易于扩张。本研究通过超微结构和生化方法分析了子宫颈胶原基质的变化。经尿素处理的子宫颈切片中的胶原纤维肿胀且松散，在横截面和纵截面中均显示出亚单位的螺旋结构。经尿素处理的组织中钌红阳性物质的正常定位缺失。对孵育后的子宫颈组织进行化学分析表明，总糖胺聚糖和硫酸皮肤素减少，后者释放到培养基中。有人认为，尿素通过溶解某种糖胺聚糖（可能是硫酸皮肤素）来解离胶原间的连接。这种溶解后，胶原纤维会被促使展开，分解呈螺旋状排列的胶原微纤维。
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