Basuyau J P, Le Bihan G, Brunelle P, Bourreille J, Bohuon C
Ann Biol Clin (Paris). 1980;38(2):123-8.
The low serum concentration of ADH requires extraction prior to assay. Various methods are compared (direct extraction by acetone, adsorption on talcum powder. Florisil, silicilic acid, Fuller's earth, QUSO, carbon-dextran, adsormone, then elution with hydrochloric acetone). The technic finally used was extraction with Florisil. The antibody used was commercially accessible. The separation of bound from free hormone required a second antibody fixed on Sephadex (DASP). The usual values found in 31 normal subjects from 22 to 89 years were: 7,4 . 4,1 pmol/l (M 2 sigma) 1 pmol/l = 1,08 pg/ml. These assays were carried out on tissue extracts prepared by mashing tumour fragments or lymph nodes removed from a patient with the Schwartz Bartter syndrome. They showed the existence in these tissues of high concentrations of ADH immunologically identical with that present in the serum of normal subjects.
抗利尿激素血清浓度较低，检测前需进行提取。对多种方法进行了比较（用丙酮直接提取、在滑石粉、弗罗里硅土、硅酸、富勒土、QUSO、碳葡聚糖、吸附激素上吸附，然后用盐酸丙酮洗脱）。最终采用的技术是用弗罗里硅土提取。所用抗体可从市场购得。游离激素与结合激素的分离需要用固定在葡聚糖凝胶上的第二抗体（双抗体固相法）。22至89岁的31名正常受试者的常规值为：7.4±4.1皮摩尔/升（均值±2标准差），1皮摩尔/升 = 1.08皮克/毫升。这些检测是在通过研磨从施瓦茨 - 巴特综合征患者身上切除的肿瘤碎片或淋巴结制备的组织提取物上进行的。结果显示这些组织中存在高浓度的抗利尿激素，其免疫学性质与正常受试者血清中的相同。
