Jaeger E, Thamm P, Knof S, Wünsch E
Hoppe Seylers Z Physiol Chem. 1978 Dec;359(12):1629-36.
Besides Nin-tert-butylated tryptophan derivatives, other tert-butyl-tryptophan analoga are also found as a result of side-reactions accurring during the acidolytic cleavage of protecting groups being based on a tert-butyl moiety. C-mono-substitution could be detected as well as di- and tri-substitution. All these tert-butylated tryptophans could be isolated in a pure form from the tert-butylation mixture either free or as derivatives and could unequivocally be identified in particular by means of mass spectrometry and nuclear magnetic resonance spectroscopy.
除了N-叔丁基化色氨酸衍生物外，在基于叔丁基部分的保护基团酸解裂解过程中发生的副反应还产生了其他叔丁基色氨酸类似物。可以检测到C-单取代以及二取代和三取代。所有这些叔丁基化色氨酸都可以以游离形式或作为衍生物从叔丁基化混合物中以纯形式分离出来，并且尤其可以通过质谱和核磁共振光谱明确鉴定。
