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短期无钙灌注后分离的大鼠肝脏的研究结果与分离的细胞相关吗?

Are findings with isolated rat livers after short calcium free perfusion relevant for isolated cells?

作者信息

Höke H, Krell H, Pfaff E

出版信息

Arch Toxicol. 1980 Mar;44(1-3):23-30. doi: 10.1007/BF00303180.

DOI:10.1007/BF00303180
PMID:7387400
Abstract

In the isolated liver transient perfusion without calcium results in cholestasis which was characterized by an increased efflux rate across the sinusoidal membrane and inhibition of the concentrative transport of bromosulfophthalein to the canalicular side of the cell. Cholestasis could not be reversed within the usual duration of liver perfusion.

摘要

在离体肝脏的无钙短暂灌注中,会导致胆汁淤积,其特征是跨窦状隙膜的流出率增加以及溴磺酞向细胞胆小管侧的浓缩转运受到抑制。在肝脏灌注的通常持续时间内,胆汁淤积无法逆转。

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Are findings with isolated rat livers after short calcium free perfusion relevant for isolated cells?短期无钙灌注后分离的大鼠肝脏的研究结果与分离的细胞相关吗?
Arch Toxicol. 1980 Mar;44(1-3):23-30. doi: 10.1007/BF00303180.
2
Characterization of calcium deprivation-induced cholestasis in the perfused rat liver.灌注大鼠肝脏中钙缺乏诱导胆汁淤积的特征分析
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引用本文的文献

1
Drug-induced intrahepatic cholestasis: characterization of different pathomechanisms.药物性肝内胆汁淤积:不同发病机制的特征
Arch Toxicol. 1987;60(1-3):124-30. doi: 10.1007/BF00296964.

本文引用的文献

1
High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study.大鼠肝脏实质细胞分离的高效制备:生化与精细结构研究
J Cell Biol. 1969 Dec;43(3):506-20. doi: 10.1083/jcb.43.3.506.
2
Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues.用于定量测定纳克级总谷胱甘肽和氧化型谷胱甘肽的酶法:应用于哺乳动物血液及其他组织
Anal Biochem. 1969 Mar;27(3):502-22. doi: 10.1016/0003-2697(69)90064-5.
3
Interaction of mixed-function oxidation with biosynthetic processes. 1. Inhibition of gluconeogenesis by aminopyrine in perfused rat liver.
Eur J Biochem. 1973 Sep 21;38(1):64-72. doi: 10.1111/j.1432-1033.1973.tb03034.x.
4
Hydroperoxide-metabolizing systems in rat liver.大鼠肝脏中的过氧化氢代谢系统。
Eur J Biochem. 1975 Sep 15;57(2):503-12. doi: 10.1111/j.1432-1033.1975.tb02325.x.
5
Excretion of taurocholate from isolated hepatocytes.从分离的肝细胞中排泄牛磺胆酸盐。
Eur J Biochem. 1976 Dec 11;71(2):369-73. doi: 10.1111/j.1432-1033.1976.tb11123.x.
6
Determination of the biliary tree capacity of the rat in its distended and nondistended state using 35 S-sulfobromophtalein glutathione (35 S-BSP-GSH) as a marker.
J Physiol (Paris). 1977 Jun;73(1):37-46.
7
Isolated rat liver needs calcium to make bile.离体大鼠肝脏需要钙来生成胆汁。
Proc Soc Exp Biol Med. 1977 Jul;155(3):314-7. doi: 10.3181/00379727-155-39797.
8
Transport and metabolism of bromosulfophthalein by isolated rat liver cells.溴磺酞在分离的大鼠肝细胞中的转运与代谢
Eur J Biochem. 1979 Mar;94(2):617-22. doi: 10.1111/j.1432-1033.1979.tb12932.x.
9
Criteria of viability of isolated liver cells.分离肝细胞的活力标准。
Hoppe Seylers Z Physiol Chem. 1975 Jun;356(6):827-38. doi: 10.1515/bchm2.1975.356.s1.827.
10
Uptake of taurocholic acid into isolated rat-liver cells.牛磺胆酸在分离的大鼠肝细胞中的摄取。
Eur J Biochem. 1975 Jul 15;55(3):617-23. doi: 10.1111/j.1432-1033.1975.tb02199.x.