采用酶联免疫吸附测定法（ELISA）检测人支气管癌中的肿瘤相关抗原。
Detection of tumour-associated antigens in human bronchogenic carcinoma by the enzyme-linked immunosorbent assay (ELISA).
作者信息
Kelly B S, Levy J G
出版信息
Br J Cancer. 1980 Mar;41(3):388-98. doi: 10.1038/bjc.1980.62.
An enzyme-linked immunosorbent assay (ELISA) was developed in which a tumour-specific component of human squamous-cell carcinoma of the lung could be readily detected using an absorbed rabbit antiserum. This antiserum did not react with equivalent preparations made from pooled normal lung tissue. In a study using the coded sera from normal individuals and preoperative patients subsequently shown to have Stage I bronchogenic carcinoma of various histological types, we found that the patients' sera effectively inhibited the reaction between the rabbit antiserum and the partially purified tumour antigen, whereas the serum from normal individuals did not.
开发了一种酶联免疫吸附测定法（ELISA），使用吸收的兔抗血清可以很容易地检测到人肺鳞状细胞癌的肿瘤特异性成分。这种抗血清与从正常肺组织池中制备的等效制剂不发生反应。在一项研究中，使用来自正常个体和随后被证明患有各种组织学类型的I期支气管源性癌的术前患者的编码血清，我们发现患者的血清有效地抑制了兔抗血清与部分纯化的肿瘤抗原之间的反应，而正常个体的血清则没有。
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