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使用免疫吸附柱分离与人类支气管源性癌相关抗原的特异性抗体。

The use of immunoadsorbent columns for the isolation of antibodies specific for antigens associated with human bronchogenic carcinoma.

作者信息

Watson R D, Smith A G, Levy J G

出版信息

Br J Cancer. 1974 Mar;29(3):183-8. doi: 10.1038/bjc.1974.57.

DOI:10.1038/bjc.1974.57
PMID:4208459
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2009101/
Abstract

An immunoadsorbent technique is described whereby tumour-specific antibodies may be isolated. Extracts from normal human lung tissue were pooled and bound to cyanogen bromide activated Sepharose 4B. Antisera raised in rabbits to a variety of extracts from human bronchogenic carcinoma were passed through these immunoadsorbent columns to yield antisera specific for tumour-associated antigens as demonstrated by immunodiffusion and immunoelectrophoresis.

摘要

本文描述了一种免疫吸附技术，通过该技术可分离肿瘤特异性抗体。将正常人肺组织提取物汇集并结合到溴化氰活化的琼脂糖4B上。用多种人支气管源性癌提取物免疫兔产生的抗血清通过这些免疫吸附柱，通过免疫扩散和免疫电泳证明可产生针对肿瘤相关抗原的特异性抗血清。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0265/2009101/221470d473e0/brjcancer00336-0007-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0265/2009101/bd4b905c0431/brjcancer00336-0006-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0265/2009101/9af8295a622e/brjcancer00336-0005-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0265/2009101/221470d473e0/brjcancer00336-0007-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0265/2009101/bd4b905c0431/brjcancer00336-0006-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0265/2009101/9af8295a622e/brjcancer00336-0005-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0265/2009101/221470d473e0/brjcancer00336-0007-a.jpg

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The use of immunoadsorbent columns for the isolation of antibodies specific for antigens associated with human bronchogenic carcinoma.使用免疫吸附柱分离与人类支气管源性癌相关抗原的特异性抗体。
Br J Cancer. 1974 Mar;29(3):183-8. doi: 10.1038/bjc.1974.57.
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引用本文的文献

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A human tumour-associated membrane antigen from squamous-cell carcinoma of the lung.一种来自肺鳞状细胞癌的人类肿瘤相关膜抗原。
Br J Cancer. 1980 May;41(5):705-15. doi: 10.1038/bjc.1980.132.
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Br Med J. 1975 Aug 23;3(5981):461-4. doi: 10.1136/bmj.3.5981.461.
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本文引用的文献

1
DEMONSTRATION OF TUMOR-SPECIFIC ANTIGENS IN HUMAN COLONIC CARCINOMATA BY IMMUNOLOGICAL TOLERANCE AND ABSORPTION TECHNIQUES.通过免疫耐受和吸收技术证明人类结肠癌中的肿瘤特异性抗原
J Exp Med. 1965 Mar 1;121(3):439-62. doi: 10.1084/jem.121.3.439.
2
Chemical coupling of proteins to agarose.蛋白质与琼脂糖的化学偶联。
Nature. 1967 Sep 30;215(5109):1491-2. doi: 10.1038/2151491a0.
3
Salt extraction of soluble HL-A antigens.可溶性HL-A抗原的盐提取法。
West J Med. 1977 Feb;126(2):135-7.
4
Immunological responsiveness and adjunct immunotherapy in lung cancer.肺癌中的免疫反应性及辅助免疫治疗
Jpn J Surg. 1978 Dec;8(4):300-7. doi: 10.1007/BF02469412.
5
Extraction and preliminary characterization of a human bronchogenic carcinoma antigen.一种人支气管源性癌抗原的提取及初步鉴定
Br J Cancer. 1975 Apr;31(4):379-86. doi: 10.1038/bjc.1975.77.
6
The detection by immunodiffusion of tumour associated antigenic components in extracts of human bronchongenic carcinoma.通过免疫扩散法检测人支气管源性癌提取物中的肿瘤相关抗原成分。
Br J Cancer. 1975 Sep;32(3):300-9. doi: 10.1038/bjc.1975.228.
Science. 1971 Jun 11;172(3988):1134-6. doi: 10.1126/science.172.3988.1134.
4
Serum-mediated protection of neoplastic cells from inhibition by lymphocytes immune to their tumor-specific antigens.血清介导的肿瘤细胞免受对其肿瘤特异性抗原具有免疫性的淋巴细胞抑制的保护作用。
Proc Natl Acad Sci U S A. 1969 Feb;62(2):362-8. doi: 10.1073/pnas.62.2.362.
5
Immunologic studies of human serous cystadenocarcinoma of ovary. Demonstration of tumor-associated antigens.卵巢浆液性囊腺癌的免疫学研究。肿瘤相关抗原的证明。
Cancer. 1973 Mar;31(3):588-95. doi: 10.1002/1097-0142(197303)31:3<588::aid-cncr2820310314>3.0.co;2-e.
6
Antigen solubilized from human solid tumours: lymphocyte stimulation and cutaneous delayed hypersensitivity.从人类实体瘤中溶解的抗原：淋巴细胞刺激与皮肤迟发型超敏反应。
Nat New Biol. 1973 Jun 6;243(127):188-90. doi: 10.1038/newbio243188a0.
7
Antigen solubilized from human leukemia: lymphocyte stimulation.从人白血病中溶解的抗原：淋巴细胞刺激。
Science. 1972 Sep 22;177(4054):1114-5. doi: 10.1126/science.177.4054.1114.
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