Kelly B, Levy J G
Br J Cancer. 1977 Jun;35(6):828-33. doi: 10.1038/bjc.1977.125.
Xeno-antiserum specific for antigenic components of bronchogenic carcinoma was raised in rabbits, by passively immunizing them to normal human lung antigens at the same time as immunization with a tumour extract from a squamous-cell carcinoma. Antiserum so raised contained minimal quantities of anti-normal antibody which could be removed by a single absorption with glutaral-dehyde-insolubilized normal lung extract. When tested by quantitative complement fixation with a panel of tumour extracts from surgical specimens, it was found that the antiserum gave positive complement fixation with all squamous-cell carcinoma extracts tested, and with some of the extracts from bronchogenic carcinomas of differing pathological types. The antiserum was essentially negative for pooled extracts from normal lung, liver and spleen but gave a weak positive reaction with an extract of pooled foetal lung tissue.
通过在给兔子注射鳞状细胞癌肿瘤提取物的同时，被动免疫其正常人类肺抗原，制备出了对支气管源性癌抗原成分具有特异性的异种抗血清。如此制备的抗血清含有少量的抗正常抗体，可通过用戊二醛不溶性正常肺提取物单次吸收将其去除。当用一组手术标本的肿瘤提取物进行定量补体结合试验时，发现该抗血清与所有测试的鳞状细胞癌提取物以及一些不同病理类型的支气管源性癌提取物发生阳性补体结合。该抗血清对正常肺、肝和脾的混合提取物基本呈阴性，但与胎儿肺组织混合提取物有弱阳性反应。
