Frankel A D, Jones R F
Biochim Biophys Acta. 1980 Jun 19;630(2):157-64. doi: 10.1016/0304-4165(80)90417-1.
The patterns of alanine dehydrogenase, glutamate dehydrogenase and malate dehydrogenase activity were studied during the normal vegetative cell cycle and during the processes of gametic differentiation and dedifferentiation in synchronized cultures of Chlamydomonas reinhardtii. During all three phases of growth and differentiation the synthesis of DNA was also measured. During gametic differentiation all three enzyme levels were suppressed compared to vegetative cells although DNA and cell number were comparable. During gametic dedifferentiation no DNA synthesis occurred during the first 24 h cycle and only a doubling during the second. It was not until the third cycle that a normal 4-fold increase in DNA was observed. Cell number followed a similar pattern. Although the levels of alanine dehydrogenase and malate dehydrogenase were uniformly low during the first cycle when glutamate dehydrogenase increased 4-fold, during the second cycle the patterns of these enzymes changed markedly. The enzymes did not attain levels characteristic of vegetative cells until the third cycle.
在莱茵衣藻同步培养物的正常营养细胞周期以及配子分化和去分化过程中，研究了丙氨酸脱氢酶、谷氨酸脱氢酶和苹果酸脱氢酶的活性模式。在生长和分化的所有三个阶段，还测量了DNA的合成。在配子分化过程中，与营养细胞相比，尽管DNA和细胞数量相当，但所有三种酶的水平均受到抑制。在配子去分化过程中，第一个24小时周期内没有发生DNA合成，第二个周期仅加倍。直到第三个周期才观察到DNA正常增加4倍。细胞数量遵循类似的模式。尽管在第一个周期中丙氨酸脱氢酶和苹果酸脱氢酶的水平一直很低，而谷氨酸脱氢酶增加了4倍，但在第二个周期中，这些酶的模式发生了明显变化。直到第三个周期，这些酶才达到营养细胞的特征水平。
