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Flash-induced kinetic infrared spectroscopy applied to biochemical systems.

作者信息

Siebert F, Mäntele W, Kreutz W

出版信息

Biophys Struct Mech. 1980;6(2):139-46. doi: 10.1007/BF00535750.

DOI:10.1007/BF00535750
PMID:7388122
Abstract

A flash photolysis apparatus with monitoring infrared beam is described allowing measurements of relative transmission changes of 10(-3) in times of a few milliseconds. The investigation of the photodissociation of CO-myoglobin confirms the results obtained by static infrared difference spectroscopy. The application of our method to the rhodopsin/Meta II transition reveals signals which can tentatively be ascribed to the disappearance of the C = C-band of the protonated N-retinylidene Schiff base in rhodopsin. The developed method will be compared with other existing methods of kinetic vibronic spectroscopy such as kinetic resonance Raman spectroscopy and kinetic Fourier infrared spectroscopy.

摘要

相似文献

1
Flash-induced kinetic infrared spectroscopy applied to biochemical systems.
Biophys Struct Mech. 1980;6(2):139-46. doi: 10.1007/BF00535750.
2
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4
Fourier-transform infrared difference spectroscopy of rhodopsin and its photoproducts at low temperature.视紫红质及其光产物在低温下的傅里叶变换红外差光谱
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Absolute absorption spectra of batho- and photorhodopsins at room temperature. Picosecond laser photolysis of rhodopsin in polyacrylamide.室温下嗜盐视紫红质和光视紫红质的绝对吸收光谱。视紫红质在聚丙烯酰胺中的皮秒激光光解。
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Investigations of the rhodopsin/Meta I and rhodopsin/Meta II transitions of bovine rod outer segments by means of kinetic infrared spectroscopy.通过动力学红外光谱法对牛视杆外段视紫红质/视紫红质中间体I和视紫红质/视紫红质中间体II转变的研究。
Biophys Struct Mech. 1980;6(2):147-64. doi: 10.1007/BF00535751.
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Investigations of the rhodopsin/Meta I and rhodopsin/Meta II transitions of bovine rod outer segments by means of kinetic infrared spectroscopy.通过动力学红外光谱法对牛视杆外段视紫红质/视紫红质中间体I和视紫红质/视紫红质中间体II转变的研究。
Biophys Struct Mech. 1980;6(2):147-64. doi: 10.1007/BF00535751.
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Effect of selected anions and solvents on the electron absorption, nuclear magnetic resonance, and infrared spectra of the N-retinylidene-n-butylammonium cation.特定阴离子和溶剂对N-视黄叉基正丁基铵阳离子的电子吸收光谱、核磁共振光谱和红外光谱的影响
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7
Rapid-flow resonance Raman spectroscopy of photolabile molecules: rhodopsin and isorhodopsin.光不稳定分子的快速流动共振拉曼光谱：视紫红质和异视紫红质。
Proc Natl Acad Sci U S A. 1976 Jan;73(1):1-5. doi: 10.1073/pnas.73.1.1.
8
Resonance Raman kinetic spectroscopy of bacteriorhodopsin on the microsecond time scale.在微秒时间尺度上对细菌视紫红质进行共振拉曼动力学光谱分析。
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Proteins of bovine rod outer segments characterized by SDS-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳对牛视杆细胞外段的蛋白质进行表征。
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