Hocking W G, Golde D W
Blood. 1980 Jul;56(1):118-24.
Pronlonged in vitro growth of murine bone marrow has been achieved using a flask culture system. We report the adaption of the technique to the growth of human bone marrow. In this system, CFU-GM and CFU-E are maintained for 4-9 wk. Morphologically recognizable granulopoiesis occurs for 4-6 wk and erythropoiesis for 1.5-2.5 wk. Functional T lymphocytes are maintained for at least 5 wk. The adherent population is composed of macrophages, fibroblast-like cells, and flat pavement-like cells. Fat-containing cells are not prominant. This culture system provides a means to study human hematopoietic cell proliferation and differentiation in vitro, as well as to examine interactions between stromal cells and hematopoietic and lymphoid progenitors.
利用烧瓶培养系统已实现小鼠骨髓的长期体外生长。我们报告了该技术对人骨髓生长的适应性。在这个系统中，CFU-GM和CFU-E可维持4至9周。形态学上可识别的粒细胞生成持续4至6周，红细胞生成持续1.5至2.5周。功能性T淋巴细胞至少维持5周。贴壁细胞群体由巨噬细胞、成纤维细胞样细胞和平铺的扁平细胞组成。含脂肪的细胞不突出。这种培养系统提供了一种体外研究人类造血细胞增殖和分化的方法，以及研究基质细胞与造血及淋巴祖细胞之间相互作用的方法。
