Clarke E, McCann S R
Dept. of Haematology, St. James Hospital, Dublin, Ireland.
Eur J Haematol. 1991 May;46(5):296-300. doi: 10.1111/j.1600-0609.1991.tb01542.x.
The absence of a pluripotent stem cell assay for human cells means that we must rely on assays of committed progenitors as a means for examining the ability of long-term bone marrow culture (LTBMC) to support haemopoiesis. The CFU-GM assay has been employed by many authors to demonstrate that the proliferative capacity and differentiation potential has been retained in LTBMC. In this study the non-adherent cells from human LTBMC were set up using a liquid culture technique for the clonal proliferation of stromal colonies (CFU-F). Weekly plating of the non-adherent cells produced CFU-F for up to 7 wk. Morphological, cytochemical and antigenic characterisation of these colonies revealed that they were identical to those grown from bone marrow de novo. LTBMC can now be used to identify the interactions between the haemopoietic inductive microenvironment and stromal progenitors and allow us to investigate the precise role of cell-cell interactions or cytokine influences on the proliferative capacity of stromal cell progenitors.
由于缺乏针对人类细胞的多能干细胞检测方法，我们必须依靠定向祖细胞检测，以此来检验长期骨髓培养（LTBMC）支持造血的能力。许多作者采用CFU-GM检测方法来证明LTBMC中增殖能力和分化潜能得以保留。在本研究中，利用液体培养技术对人LTBMC中的非贴壁细胞进行培养，以实现基质集落（CFU-F）的克隆增殖。每周接种非贴壁细胞，可产生CFU-F长达7周。对这些集落进行形态学、细胞化学和抗原特性分析，结果显示它们与直接从骨髓生长的集落相同。现在，LTBMC可用于识别造血诱导微环境与基质祖细胞之间的相互作用，并使我们能够研究细胞间相互作用或细胞因子对基质细胞祖细胞增殖能力的精确影响。
