大鼠肾激肽释放酶的纯化及其与大鼠尿激肽释放酶的化学关系。

Purification of renal rat kallikrein and chemical relations with urinary rat kallikrein.

作者信息

Porcelli G, Marini-Bettolo G B, Croxatto H R, Di Torio M, Micotti G

出版信息

Ital J Biochem. 1978 Jul-Aug;27(4):201-10.

Abstract

A highly purified kallikrein from rat kidney was obtained by a multi-step purification procedure including gel filtration on Sephadex G-100, chromatography on Whatman CM-32 and DE-32 celluloses and Sephadex G-100 superfine. Molecular weight of 40,000 was estimated by sodium dodecyl sulfate polyacrylamide disc gel electrophoresis. On the purified enzyme the aminoacid analysis was determined. Some chemical differences between renal kallikrein and the most abundant rat urinary kallikrein were illustrated.

摘要

通过多步纯化程序，包括在Sephadex G - 100上进行凝胶过滤、在Whatman CM - 32和DE - 32纤维素以及Sephadex G - 100超细凝胶上进行色谱分离，从大鼠肾脏中获得了高度纯化的激肽释放酶。通过十二烷基硫酸钠聚丙烯酰胺圆盘凝胶电泳估计其分子量为40,000。对纯化后的酶进行了氨基酸分析。阐明了肾激肽释放酶与大鼠尿液中含量最丰富的激肽释放酶之间的一些化学差异。