Hattori M, Yoshimoto Y, Matsukura S, Fujita T
Cancer. 1980 Jul 15;46(2):355-61. doi: 10.1002/1097-0142(19800715)46:2<355::aid-cncr2820460224>3.0.co;2-u.
Human chorionic gonadotropin (hCG) and its subunits in plasma, urine, ascites, and tumor extracts from four patients with hCG-producing tumors (undifferentiated cell carcinoma and choriocarcinoma of the bladder, malignant teratoma of the retroperitoneum, and pancreatic carcinoma) were measured by the radioimmunoassays specific to each component. While both free alpha and beta subunits as well as the whole molecule of hCG were found in all these samples, the proportion of beta subunit was much higher in these tumor extracts than in the placental extracts. Since the alpha and beta subunits are known to be translated from separate mRNAs, such increase of subunit is probably due to the predominant increase of mRNA encoding beta subunit of hCG in the hCG-producing tumors. Gel filtration of the extracts of these four tumors on Sephadex G-100 and G-150 columns demonstrated a heterogeneity of hCG, alpha, and beta subunits in these tumor extracts, and the elution profiles of plasma, urine and tumor extracts were slightly different. Lower molecular weight forms of hCG and its beta subunit were present in all urine samples. The significance of these heterogeneities remains to be elucidated.
采用针对各成分的放射免疫分析法，对4例产生人绒毛膜促性腺激素（hCG）的肿瘤（膀胱未分化细胞癌和绒毛膜癌、腹膜后恶性畸胎瘤和胰腺癌）患者的血浆、尿液、腹水及肿瘤提取物中的hCG及其亚基进行了检测。在所有这些样本中均发现了游离的α亚基和β亚基以及完整的hCG分子，但这些肿瘤提取物中β亚基的比例远高于胎盘提取物。由于已知α亚基和β亚基是由不同的mRNA翻译而来，这种亚基比例的增加可能是由于产生hCG的肿瘤中编码hCGβ亚基的mRNA显著增加所致。对这4种肿瘤的提取物在Sephadex G - 100和G - 150柱上进行凝胶过滤，结果表明这些肿瘤提取物中的hCG、α亚基和β亚基具有异质性，血浆、尿液和肿瘤提取物的洗脱图谱略有不同。所有尿液样本中均存在较低分子量形式的hCG及其β亚基。这些异质性的意义尚待阐明。
