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红细胞膜隆起的外观。冷却速率的影响。

The appearance of erythrocyte membrane elevations. Effects of cooling rates.

作者信息

Goekoop J G, Spies F, Wisse D M, de Vries E, Verkleij A J, van Kempen G M

出版信息

Cell Biol Int Rep. 1980 Jan;4(1):37-42. doi: 10.1016/0309-1651(80)90007-7.

Abstract

Low cooling rates during the freezing procedure of normal human blood reveals red cell membrane elevations in freeze-etch electron microscopy. When high cooling rate is applied, these morphological changes are present, if the blood samples are quenched from 5 degrees C. The number of elevations is strongly reduced by low pH and glycerol. Elevations are not observed in ghosts. The formation of intramembrane particle aggregation is differently affected by many conditions.

摘要

正常人血液冷冻过程中的低冷却速率在冷冻蚀刻电子显微镜下显示红细胞膜隆起。当采用高冷却速率时,如果血液样本从5摄氏度骤冷,这些形态变化就会出现。低pH值和甘油会使隆起的数量大幅减少。在血影中未观察到隆起。膜内颗粒聚集的形成受多种条件的影响各不相同。

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pH-dependent behaviour of erythrocyte membrane elevations.红细胞膜隆起的pH依赖性行为。
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X-ray diffraction and electron microscopy studies of frozen erythrocyte membrane preparations.
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