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单份血清样本中维生素D主要代谢物的定量分析。II. 紫外吸收法和竞争性蛋白结合分析法测定

Quantitation of the main metabolites of vitamin D in a single serum sample. II. Determination by UV-absorption and competitive protein binding assays.

作者信息

Aksnes L

出版信息

Clin Chim Acta. 1980 Jun 10;104(2):147-59. doi: 10.1016/0009-8981(80)90191-6.

DOI:10.1016/0009-8981(80)90191-6
PMID:7389129
Abstract

The present report, together with extraction, separation and purification procedures described previously [1], constitutes a sensitive method for determination of vitamin D and its main metabolites in 2 ml of human serum. By omitting vitamin D itself, 25-OHD, 24,25-(OH)2D, 25,26-(OH)2D and 1,25-(OH)2D could be determined in less than 0.5 ml serum. The quantitation methods described have detection limits of 1.8 pmol for vitamin D, 20 fmol for 25-OHD, 24,25-(OH)2D and 25,26-(OH)2D, and 1 fmol for 1,25-(OH)2D.

摘要

本报告与先前所述的提取、分离和纯化程序[1]一起，构成了一种用于测定2ml人血清中维生素D及其主要代谢物的灵敏方法。通过省略维生素D本身，可在少于0.5ml血清中测定25-OHD、24,25-(OH)2D、25,26-(OH)2D和1,25-(OH)2D。所述定量方法的检测限为：维生素D为1.8pmol，25-OHD、24,25-(OH)2D和25,26-(OH)2D为20fmol，1,25-(OH)2D为1fmol。

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Quantitation of the main metabolites of vitamin D in a single serum sample. II. Determination by UV-absorption and competitive protein binding assays.单份血清样本中维生素D主要代谢物的定量分析。II. 紫外吸收法和竞争性蛋白结合分析法测定
Clin Chim Acta. 1980 Jun 10;104(2):147-59. doi: 10.1016/0009-8981(80)90191-6.
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Clin Chim Acta. 1980 Jun 10;104(2):133-46. doi: 10.1016/0009-8981(80)90190-4.
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