Budd R D, Leung W J
Clin Toxicol. 1980 Mar;16(1):55-9. doi: 10.3109/15563658008989924.
A thin-layer chromatographic method for the simultaneous screening and confirmation of methadone and its primary metabolite (2-ethylidene-1,5-diemthyl-3,3-diphenylpyrrolidine) in urine specimens is presented. Urine is made basic and extracted with organic solvent. After separation and concentration, the residue is spotted, half on each of two thin-layer chromatography plates. One plate is developed in an ethyl acetate:methanol:diethylamine (90:10:1.6) solvent system and the second in an ethyl acetate:methylene chloride:propylamine (85:17:1.0) solvent system. Methadone and its primary metabolite can be detected in concentration as low as 0.5 micrograms/mL without interference from other drugs and urinary substances.
本文介绍了一种用于同时筛查和确证尿液样本中吗啡及其主要代谢物（2-亚乙基-1,5-二甲基-3,3-二苯基吡咯烷）的薄层色谱法。将尿液碱化并用有机溶剂萃取。分离并浓缩后，将残渣点样，分别点在两块薄层色谱板上。一块板在乙酸乙酯：甲醇：二乙胺（90:10:1.6）溶剂系统中展开，另一块在乙酸乙酯：二氯甲烷：丙胺（85:17:1.0）溶剂系统中展开。吗啡及其主要代谢物的检测浓度低至0.5微克/毫升，且不受其他药物和尿液物质的干扰。
