Budd R D, Mathis D F, Leung W J
Clin Toxicol. 1980 Mar;16(1):61-6. doi: 10.3109/15563658008989925.
A thin-layer chromatography (TLC) procedure for the screening and confirmation of urinary codeine and morphine has been developed. Urine samples are hydrolyzed to liberate free codeine and morphine, extracted into an organic solvent, and concentrated. For screening, the extraction residues are spotted on TLC plates which are developed in chloroform:ethyl acetate:methanol:propylamine (35:45:5:5). For confirmation, the extraction residues are acetylated and then spotted on TLC plates which are developed in hexane:chloroform:diethylamine (50:30:7). The spots for the two drugs are visualized with acidified iodoplatinate. Codeine and morphine are well separated from one another and from normal urinary substances on both plates and can be detected at a concentration of 0.4 micrograms/mL.
已开发出一种用于筛查和确证尿中可待因和吗啡的薄层色谱（TLC）方法。尿样经水解以释放游离可待因和吗啡，用有机溶剂萃取并浓缩。用于筛查时，将萃取残渣点在TLC板上，在氯仿：乙酸乙酯：甲醇：丙胺（35:45:5:5）中展开。用于确证时，将萃取残渣乙酰化，然后点在TLC板上，在己烷：氯仿：二乙胺（50:30:7）中展开。两种药物的斑点用酸化碘铂酸盐显色。可待因和吗啡在两块板上均能与彼此以及正常尿液物质良好分离，且在浓度为0.4微克/毫升时即可检测到。
