Helbing G, Burri C, Heit W, Neugebauer R, Rüter A
Chir Forum Exp Klin Forsch. 1980:47-51.
Long-term results after cartilage transplantation are not satisfactory owing to poor viability of the chondrocytes. In vitro experiments with isolated fetal and neonatal rabbit chondrocytes revealed a much better proliferative ability compared with adult cartilage derived cells. Cryopreservation of cell suspensions did not decrease the proliferative ability. In in vivo experiments, cultured neonatal, fetal, and cryopreserved rabbit chondrocytes were tested in transplants and compared with cell-free controls or radiated chondrocytes. These chondrocytic transplants showed good viability and seemed to synthesize cartilage matrix, which could be stained with safranin, whereas only fibrous tissue was found in controls.
由于软骨细胞的生存能力较差，软骨移植后的长期效果并不理想。对分离出的兔胎儿和新生软骨细胞进行的体外实验表明，与成年软骨来源的细胞相比，其增殖能力要强得多。细胞悬液的冷冻保存并没有降低其增殖能力。在体内实验中，将培养的新生、胎儿和冷冻保存的兔软骨细胞用于移植实验，并与无细胞对照组或经辐射的软骨细胞进行比较。这些软骨细胞移植显示出良好的生存能力，似乎能够合成可被番红染色的软骨基质，而在对照组中只发现了纤维组织。
