大鼠脊髓中甲硫氨酸脑啡肽免疫反应性物质在体内的释放。

Release of methionine-enkephalin immunoreactivity from the rat spinal cord in vivo.

作者信息

Yaksh T L, Elde R P

出版信息

Eur J Pharmacol. 1980 May 16;63(4):359-62. doi: 10.1016/0014-2999(80)90266-6.

Abstract

The spinal cord was superfused in situ, in the chloralose-urethanized rat. Radioimmunoassay for methionine-enkephalin-like activity revealed that the basal levels of release were on the order of 0.4 ng/ml/10 min. Addition of potassium (40 mM) to the perfusate yielded a 3-fold increase in the levels of MEI. This increase was blocked by the substitution of cobalt for calcium in the superfusate. These data suggest that MEI activity in the cord is stored in a releasible fraction and provides in vivo support for the concept that methionine-enkephalin is a neurotransmitter in the cord.

摘要

在水合氯醛-氨基甲酸乙酯麻醉的大鼠中，对脊髓进行原位灌流。甲硫氨酸脑啡肽样活性的放射免疫测定显示，基础释放水平约为0.4 ng/ml/10分钟。向灌流液中添加钾（40 mM）可使甲硫氨酸脑啡肽样免疫反应物（MEI）水平增加3倍。用钴替代灌流液中的钙可阻断这种增加。这些数据表明，脊髓中的MEI活性存在于可释放部分中，并为甲硫氨酸脑啡肽是脊髓中的一种神经递质这一概念提供了体内支持。