Slaby F, Farquhar M G
Mol Cell Endocrinol. 1980 Apr;18(1):21-32. doi: 10.1016/0303-7207(80)90004-0.
A procedure has been developed for separating rat somatotroph and mammotroph secretory granules from other membranous organelles. The approach devised is a 6-step procedure involving filtration and equilibrium and rate density-gradient centrifugation in Metrizamide gradients of low ionic strength. The use of Metrizamide minimizes disruption and solubilization of the contents of these granules. The majority of the somatotroph and mammotroph granules can be isolated in a gradient fraction virtually free of any mitochondria, microsomes or other secretory granules.
已开发出一种从其他膜性细胞器中分离大鼠生长激素分泌细胞和催乳激素分泌细胞分泌颗粒的方法。所设计的方法是一个6步程序，包括在低离子强度的甲泛葡胺梯度中进行过滤、平衡和速率密度梯度离心。使用甲泛葡胺可将这些颗粒内容物的破坏和溶解降至最低。大多数生长激素分泌细胞和催乳激素分泌细胞颗粒可在一个几乎不含任何线粒体、微粒体或其他分泌颗粒的梯度级分中分离出来。
