Kather H, Simon B
Horm Metab Res. 1980 Apr;12(4):169-72. doi: 10.1055/s-2007-996232.
Adenosine is known to modulate adenylate cyclase activity in a variety of tissues. We have tested the effects of adenosine on basal activity and the catecholamine- or prostaglandin E1-stimulated activity of the human fat cell adenylate cyclase. Adenosine caused a dose-dependent inhibition of basal enzyme activity as well as of adrenaline-and prostaglandin E1-stimulated rates of 3',5'-cyclic AMP accumulation. The adenosine-induced inhibition was specific since other nucleosides or their respective nitrogenous parent bases, with the exception of adenine, failed to mimic the action of adenosine. In addition, the adenosine-induced inhibition could be reversed by inclusion of adenosine deaminase. The results are compatible with the concept of adenosine acting as an inhibitor of lipolysis at the level of the membrane-bound adenylate cyclase. They show that this nucleoside can also inhibit the prostaglandin E1-induced stimulation of the human fat cell adenylate cyclase thereby suggesting that the effects of adenosine and prostaglandins might be antagonistic under conditions where prostaglandins act as stimulators of lipolysis.
已知腺苷可调节多种组织中的腺苷酸环化酶活性。我们测试了腺苷对人脂肪细胞腺苷酸环化酶基础活性以及儿茶酚胺或前列腺素E1刺激活性的影响。腺苷对基础酶活性以及肾上腺素和前列腺素E1刺激的3',5'-环磷酸腺苷积累速率产生剂量依赖性抑制。腺苷诱导的抑制具有特异性，因为除腺嘌呤外，其他核苷或其各自的含氮母碱基均无法模拟腺苷的作用。此外，加入腺苷脱氨酶可逆转腺苷诱导的抑制作用。这些结果与腺苷在膜结合腺苷酸环化酶水平上作为脂解抑制剂的概念相符。结果表明，这种核苷还可抑制前列腺素E1诱导的人脂肪细胞腺苷酸环化酶刺激作用，从而提示在前列腺素作为脂解刺激剂的条件下，腺苷和前列腺素的作用可能是拮抗的。
