Courtoy R, Boniver J, Simar L J
Histochemistry. 1980;66(1):49-58. doi: 10.1007/BF00493245.
The modifications of mouse mast cells incubated in solutions of polylysine at two different concentrations are studied. At higher dose (0.5 mg/ml), the polycation has evident toxic effects. At lower dose (0.5 micrograms/ml), it induces some modifications of the granule morphology. Cytochemical reactions to demonstrate polyanions, basic proteins and organic bases are used to study the granule content. A polylysine treatment promotes the release of basic groups (vaso-amines) and sulphydryl-containing compounds (basic protein) from the mast cell granules. On the contrary, polyanions remain in the cells and correspond to the granule matrix persisting after amino-liberator treatment.
研究了在两种不同浓度的聚赖氨酸溶液中孵育的小鼠肥大细胞的变化。在较高剂量（0.5毫克/毫升）时，聚阳离子具有明显的毒性作用。在较低剂量（0.5微克/毫升）时，它会诱导颗粒形态发生一些变化。利用显示多阴离子、碱性蛋白和有机碱的细胞化学反应来研究颗粒内容物。聚赖氨酸处理促进了肥大细胞颗粒中碱性基团（血管胺）和含巯基化合物（碱性蛋白）的释放。相反，多阴离子保留在细胞中，并且与经氨基释放剂处理后持续存在的颗粒基质相对应。
