Suzuki T, Goto R, Tamemasa O
J Biochem. 1980 May;87(5):1285-91. doi: 10.1093/oxfordjournals.jbchem.a132866.
Nonhistone chromasomal proteins (NHP) of Ehrlich ascites tumor cells were fractionated into four subfractions by successive extractions with 0.35 M, 0.45 M, and 0.60 M NaCl, and finally 2.0 M NaCl + 5.0 M urea. The CD spectra and transcriptional activities of complexes reconstituted from DNA, histone, and each of these four NHP subfractions obtained from Ehrlich tumor cells were measured in order to identify the effect of each NHP subfraction. The positive ellipticity in the CD of the DNA-histone complex was considerably increased when reconstituted with 0.35 M- or 0.45 M-NHP. The 0.35 M- and 0.45 M-NHP also increased the transcriptional activity of DNA-histone complex. The activities of DNA-histone-0.35 M NHP complex increased progressively with increase in the 0.35 M NHP/DNA ratio. These results suggest that there is a correlation between the increase in transcriptional activity of DNA-histone complexes with 0.35 M- or 0.45 M-NHP and the increase in the percentage of DNA in the B conformation in complexes.
用0.35M、0.45M和0.60M氯化钠，最后用2.0M氯化钠+5.0M尿素连续提取艾氏腹水瘤细胞的非组蛋白染色体蛋白（NHP），将其分为四个亚组分。为了确定每个NHP亚组分的作用，测量了由DNA、组蛋白以及从艾氏瘤细胞获得的这四个NHP亚组分中的每一个重构的复合物的圆二色光谱（CD光谱）和转录活性。当与0.35M或0.45M的NHP重构时，DNA-组蛋白复合物CD中的正椭圆率显著增加。0.35M和0.45M的NHP也增加了DNA-组蛋白复合物的转录活性。DNA-组蛋白-0.35M NHP复合物的活性随着0.35M NHP/DNA比例的增加而逐渐增加。这些结果表明，DNA-组蛋白复合物与0.35M或0.45M的NHP重构时转录活性的增加与复合物中处于B构象的DNA百分比的增加之间存在相关性。
