Hsieh S, Goldstein E, Lippert W, Margulies L
J Infect Dis. 1978 Dec;138(6):754-9. doi: 10.1093/infdis/138.6.754.
The importance of IgG attached to protein A in the initial reaction of inspired Staphylococcus aureus and alveolar macrophages was studied by infecting unimmunized mice with aerosols of S. aureus strains 566 and Wood 46 with high and low protein A content. At 2, 4, and 8 hr after infection, the presence of IgG attached to S. aureus and rates of staphylococcal ingestion and killing by macrophages were determined. IgG was detected by staining of sections of the right lung with fluorescein-labeled goat antibody to mouse IgG. For S. aureus strain 566, 25%--40% of the total number of bacteria, as determined in equivalently sized subjacent sections stained by the Brown and Brenn tissue gram stain, contained attached IgG. A few S. aureus strain Wood 46 were surrounded by dimly fluorescing complexes. Since rates of bacterial ingestion and killing were similar for both strains in this in vivo model of infection, IgG binding to protein A does not affect the bactericidal capacity of alveolar macrophages.
通过用高蛋白A含量的金黄色葡萄球菌菌株566和气溶胶以及低蛋白A含量的Wood 46菌株气溶胶感染未免疫的小鼠，研究了附着于蛋白A的IgG在吸入的金黄色葡萄球菌与肺泡巨噬细胞初始反应中的重要性。在感染后2、4和8小时，测定附着于金黄色葡萄球菌的IgG的存在情况以及巨噬细胞对葡萄球菌的摄取和杀伤率。通过用荧光素标记的山羊抗小鼠IgG抗体对右肺切片进行染色来检测IgG。对于金黄色葡萄球菌菌株566，在通过Brown和Brenn组织革兰氏染色法染色的同等大小的相邻切片中确定，总数的25% - 40%的细菌含有附着的IgG。少数Wood 46金黄色葡萄球菌菌株被微弱荧光复合物包围。由于在这种体内感染模型中两种菌株的细菌摄取和杀伤率相似，IgG与蛋白A的结合不会影响肺泡巨噬细胞的杀菌能力。
