Gospe S M, Wilson W A
J Pharmacol Exp Ther. 1980 Jul;214(1):112-8.
Burst-firing in neurosecretory cell R 15 of Aplysia californica is inhibited by the bath application of dopamine. Voltage-clamp analysis reveals a complex series of changes in the current-voltage relationship of R 15. Dopamine reduces the inward current in the region of negative slope conductance and tends to linearize the current-voltage curve. A variable increase in potassium conductance is frequently observed. The loss of inward current in the region of negative slope conductance is dose-dependent, highly reproducible and relatively resistant to desensitization, and for these reasons a valid dose-response relationship can be described. The dose-response curve is sigmoidal in shape and rises from a minimal effect near 50 microM to a maximal effect near 500 microM. This dose-response curve is useful as a standard of comparison in studies of agonists and antagonists. A protocol for the quantitative study of this system is described, and the constellation of changes in the current-voltage curve produced by dopamine is discussed.
加州海兔神经分泌细胞R15中的爆发式放电受到浴槽施加多巴胺的抑制。电压钳分析揭示了R15电流-电压关系中一系列复杂的变化。多巴胺降低了负斜率电导区域的内向电流，并倾向于使电流-电压曲线线性化。经常观察到钾电导有可变增加。负斜率电导区域内向电流的丧失是剂量依赖性的，高度可重复且相对不易脱敏，因此可以描述有效的剂量-反应关系。剂量-反应曲线呈S形，从50微摩尔附近的最小效应上升到500微摩尔附近的最大效应。该剂量-反应曲线可作为激动剂和拮抗剂研究中的比较标准。描述了该系统定量研究的方案，并讨论了多巴胺引起的电流-电压曲线变化情况。
