Hydén H, Rönnbäck L
J Neurobiol. 1978 Nov;9(6):489-92. doi: 10.1002/neu.480090608.
The S-100 protein has been localized to the neuronal plasma membranes of isolated Deiters' neurons by fluorescence microscopy using fluorescein-conjugated antiserum to the protein and by immunoelectron microscopy using peroxidase-conjugated anti-S-100 antiserum. In the present study this is shown also by incubating neurons with Sepharose 4B or methylacrylate spherules to which were coupled anti-S-100 antibodies. The specificity of the antiserum is discussed in the text. The technique described can be used to study the topography of antigenic characteristics of nerve cells by using antisera insolubilized on spherules of suitable size.
通过使用与该蛋白结合的荧光素抗血清的荧光显微镜以及使用过氧化物酶结合的抗S-100抗血清的免疫电子显微镜，已将S-100蛋白定位到分离的Deiters神经元的神经细胞质膜上。在本研究中，通过用偶联有抗S-100抗体的琼脂糖4B或甲基丙烯酸酯小球孵育神经元也证明了这一点。文中讨论了抗血清的特异性。所描述的技术可用于通过使用固定在合适大小小球上的抗血清来研究神经细胞抗原特性的拓扑结构。
