Zierold K
Microsc Acta. 1980 Mar;83(1):25-32.
The freezing velocity, the most important parameter for the quality of cryofixation of biological objects, was measured in frog liver specimens. The cooling course was found to depend on the size of the specimen, the specimen support and the cooling medium used (liquid nitrogen, supercooled nitrogen, Freon 12 and propane). The results were compared with scanning electron micrographs of freeze fractures cryofixed in the same manner: Propane yielded the highest cooling rates and, consequently, the best structural preservation. Morphologically similar results were obtained by combining Freon 12 and very small specimen supports. Generally, it can be said that the smaller both specimen and specimen support are, the higher is the freezing rate and the better the structural preservation. The findings are discussed with regard to further possibilities of improving the cryofixation of biological tissue.
在青蛙肝脏标本中测量了冷冻速度，这是生物物体冷冻固定质量的最重要参数。发现冷却过程取决于标本的大小、标本支撑物以及所使用的冷却介质（液氮、过冷氮、氟利昂12和丙烷）。将结果与以相同方式冷冻固定的冷冻断裂扫描电子显微照片进行了比较：丙烷产生的冷却速率最高，因此结构保存最佳。通过将氟利昂12与非常小的标本支撑物相结合，获得了形态学上相似的结果。一般来说，可以说标本和标本支撑物越小，冷冻速率越高，结构保存越好。针对进一步改善生物组织冷冻固定的可能性对这些发现进行了讨论。
