Maddison S E, Slemenda S B, Chandler F W, Kagan I G
J Parasitol. 1978 Dec;64(6):986-93.
Intraperitoneal transfer of viable adult worms of Schistosoma mansoni did not confer protection against a challenge infection to recipient mice. Antigens of schistosome origin were evaluated for their ability, with and without concomitantly administered nonspecific adjuvants, to stimulate protective immunity against S. mansoni. Freshly perfused ground worms or a putative membrane antigen extracted with 0.5 M KC1 from adult worms, when injected together with Corynebacterium parvum (or in a single experiment with poly [A : U]), resulted in a significant reduction in worm burden of a challenge infection with S. mansoni as compared with that of untreated controls. The membrane antigen was maintained carefully at low temperatures in buffers capable of retarding enzymatic degradation while it was being prepared.
将曼氏血吸虫活的成虫进行腹腔内转移，并未使受体小鼠获得针对攻击感染的保护作用。对血吸虫来源的抗原进行了评估，观察其在有无同时给予非特异性佐剂的情况下，刺激针对曼氏血吸虫的保护性免疫的能力。刚灌注的研磨虫体或用0.5M氯化钾从成虫中提取的一种假定的膜抗原，与短小棒状杆菌一起注射时（或在一项实验中与聚 [A:U] 一起注射），与未处理的对照组相比，导致曼氏血吸虫攻击感染的虫负荷显著降低。在制备膜抗原时，将其小心地保存在能够延缓酶降解的缓冲液中，并置于低温环境。
