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刺激-分泌偶联过程中的钙调节:细胞内钙活性的连续测量

Calcium regulation during stimulus-secretion coupling: continuous measurement of intracellular calcium activities.

作者信息

O'Doherty J, Youmans S J, Armstrong W M, Stark R J

出版信息

Science. 1980 Jul 25;209(4455):510-3. doi: 10.1126/science.7394518.

Abstract

Accurate measurements of intracellular calcium activities in salivary gland epithelial cells of the insect Phormia regina were obtained with microelectrodes in which N,N'-di(11-ethoxycarbonyl)undecyl-N,N'-4,5-tetramethyl-3,6-dioxaoctane diacid diamide wsa incorporated in a liquid membrane system. When calibrated in solutions approximating the ionic concentration of the cell interior, these microelectrodes gave rapid stable responses that were linear functions of the logarithm of calcium activities and were not affected by potassium, sodium and magnesium. Continuous monitoring of calcium activities during serotonin-induced saliva release provided direct evidence of hormonal influence on transmembrane calcium movement and spontaneous regulation of intracellular calcium by stimulated cells.

摘要

使用微电极对丽蝇唾液腺上皮细胞内的钙活性进行了精确测量,该微电极将N,N'-二(11-乙氧羰基)十一烷基-N,N'-4,5-四甲基-3,6-二氧杂辛二酸二酰胺掺入液膜系统中。当在接近细胞内离子浓度的溶液中进行校准时,这些微电极给出了快速稳定的响应,这些响应是钙活性对数的线性函数,并且不受钾、钠和镁的影响。在5-羟色胺诱导唾液分泌过程中对钙活性的连续监测,为激素对跨膜钙转运的影响以及受刺激细胞对细胞内钙的自发调节提供了直接证据。

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