Ota K, Sanaka T, Agishi T, Nakajima O
Artif Organs. 1980 May;4(2):113-5. doi: 10.1111/j.1525-1594.1980.tb03915.x.
Uremic middle molecular weight substances are obtained from the ultrafiltrate of plasma of uremic patients. Seven subfractions are isolated by chromatography using Sephadex G-15 and DEAE 25 A columns. Each subfraction is tested for its inhibitory effect upon lymphocyte blastogenesis and influence upon osmotic fragility of red blood cells. Consequently, subfractions c and d exert notable effects on both lymphocytes and red blood cells. The main constituents of subfraction d are aspartic acid, glycine, glutamine, and cysteine. It is concluded that uremic middle molecular substances have a deleterious effect upon blood cells. Therefore, hemofiltration, which is advantageous for the removal of middle molecular weight substances, is considered to be a method of choice for uremic patients.
尿毒症中分子量物质是从尿毒症患者血浆的超滤物中获得的。使用葡聚糖凝胶G - 15和二乙氨基乙基纤维素25 A柱通过色谱法分离出七个亚组分。对每个亚组分进行淋巴细胞增殖抑制作用和对红细胞渗透脆性影响的测试。结果，亚组分c和d对淋巴细胞和红细胞均有显著作用。亚组分d的主要成分是天冬氨酸、甘氨酸、谷氨酰胺和半胱氨酸。得出结论，尿毒症中分子物质对血细胞有有害作用。因此，对去除中分子量物质有利的血液滤过被认为是尿毒症患者的一种首选治疗方法。
