灌注兔心脏中肌钙蛋白I的磷酸化位点。肾上腺素的作用。
The site of phosphorylation of troponin I in the perfused rabbit heart. The effect of adrenaline.
作者信息
Moir A J, Solaro R J, Perry S V
出版信息
Biochem J. 1980 Feb 1;185(2):505-13. doi: 10.1042/bj1850505.
Abstract
- On treatment of the perfused rabbit heart with adrenaline, the total covalently bound phosphate of troponin I increased from 1.14 mol of phosphate/mol to 1.86 mol of phosphate/mol. 2. Covalently bound phosphate could be identified only in the region of the molecule of cardiac troponin I consisting of residues 1--48. 3. When 32P-labelled orthophosphate was present in the perfusion medium the phosphate at serine-20 became radioactively labelled. This residue was the only significant site of phosphorylation that could be identified. 4. The addition of adrenaline caused a 4--5-fold increase in covalently bound [32P]phosphate. Virtually all of the 32P was located at serine-20. 5. It was concluded from these studies that the extent of phosphorylation of serine-20 of cardiac troponin I increased from 30--40% in the control perfused heart to about 100% in the presence of adrenaline.
摘要
- 用肾上腺素处理灌注的兔心脏时，肌钙蛋白I的总共价结合磷酸盐从1.14摩尔磷酸盐/摩尔增加到1.86摩尔磷酸盐/摩尔。2. 共价结合的磷酸盐仅在心肌肌钙蛋白I分子中由第1 - 48位残基组成的区域被鉴定出来。3. 当灌注培养基中存在32P标记的正磷酸盐时，丝氨酸-20处的磷酸盐被放射性标记。该残基是唯一可鉴定的显著磷酸化位点。4. 肾上腺素的添加使共价结合的[32P]磷酸盐增加了4 - 5倍。几乎所有的32P都位于丝氨酸-20处。5. 从这些研究得出结论，心肌肌钙蛋白I丝氨酸-20的磷酸化程度在对照灌注心脏中从30 - 40%增加到在肾上腺素存在下约100%。
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