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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Biochem J. 1980 Nov 1;191(2):547-54. doi: 10.1042/bj1910547.

Incubation of rabbit cardiac-muscle troponin I with phosphorylase b kinase leads to the incorporation of .07-1.2 mol of Pi/mol. 2. The major site of phosphorylation is a serine residue at position 72. 3. Lesser amounts of phosphate are incorporated into threonine-138, threonine-162 and serine 20. 4. Serine-20 is the only site that contains a significant amount of phosphate before incubation with phosphorylase b kinase. 5. Unlike the situation with serine-20, the extent of phosphorylation of serine-72 and threonine-138 in the perfused rabbit heart does not change when the heart is exposed to adrenaline (4 microM).

兔心肌肌钙蛋白I与磷酸化酶b激酶一起温育会导致每摩尔掺入0.07 - 1.2摩尔的无机磷酸（Pi）。2. 磷酸化的主要位点是第72位的丝氨酸残基。3. 较少量的磷酸被掺入到苏氨酸-138、苏氨酸-162和丝氨酸20中。4. 丝氨酸-20是在与磷酸化酶b激酶温育之前唯一含有大量磷酸的位点。5. 与丝氨酸-20的情况不同，当兔心脏暴露于肾上腺素（4微摩尔）时，灌注兔心脏中丝氨酸-72和苏氨酸-138的磷酸化程度没有变化。

Biochem J. 1980 Nov 1;191(2):547-54. doi: 10.1042/bj1910547.

Incubation of rabbit cardiac-muscle troponin I with phosphorylase b kinase leads to the incorporation of .07-1.2 mol of Pi/mol. 2. The major site of phosphorylation is a serine residue at position 72. 3. Lesser amounts of phosphate are incorporated into threonine-138, threonine-162 and serine 20. 4. Serine-20 is the only site that contains a significant amount of phosphate before incubation with phosphorylase b kinase. 5. Unlike the situation with serine-20, the extent of phosphorylation of serine-72 and threonine-138 in the perfused rabbit heart does not change when the heart is exposed to adrenaline (4 microM).

兔心肌肌钙蛋白I与磷酸化酶b激酶一起温育会导致每摩尔掺入0.07 - 1.2摩尔的无机磷酸（Pi）。2. 磷酸化的主要位点是第72位的丝氨酸残基。3. 较少量的磷酸被掺入到苏氨酸-138、苏氨酸-162和丝氨酸20中。4. 丝氨酸-20是在与磷酸化酶b激酶温育之前唯一含有大量磷酸的位点。5. 与丝氨酸-20的情况不同，当兔心脏暴露于肾上腺素（4微摩尔）时，灌注兔心脏中丝氨酸-72和苏氨酸-138的磷酸化程度没有变化。