文献检索，用中文搜 PubMed

Suppr 超能文献

核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Jeacocke S A, England P J

Biochem J. 1980 Jun 15;188(3):763-8. doi: 10.1042/bj1880763.

A method was developed for the isolation of essentially pure myosin light chains from perfused rat heart. The phosphorylation of the P-light chains was estimated by hydrolysis and measurement of phosphate released, by electrophoresis in 8 M-urea and by 32P incorporation in perfusion with [32P]Pi. 2. In control perfusions there was 0.5-0.6 mol of phosphate/mol of P-light chain. This was not changed by perfusion with 5 microM-adrenaline for 10-40s. Perfusion for 1 min with medium containing 7.5 mM-CaCl2, or for 30s with medium containing 118 mM-KCl, also did not change the phosphorylation of P-light chains. 3. It is concluded that phosphorylation of P-light chains is not important in mediating the action of inotropic agents in the heart.

开发了一种从灌注大鼠心脏中分离基本纯的肌球蛋白轻链的方法。通过水解和测量释放的磷酸盐、在8M尿素中进行电泳以及在[32P]Pi灌注中进行32P掺入来估计P轻链的磷酸化。2. 在对照灌注中，每摩尔P轻链有0.5 - 0.6摩尔磷酸盐。用5微摩尔肾上腺素灌注10 - 40秒不会改变这一情况。用含7.5毫摩尔氯化钙的培养基灌注1分钟，或用含118毫摩尔氯化钾的培养基灌注30秒，也不会改变P轻链的磷酸化。3. 得出的结论是，P轻链的磷酸化在介导心脏中变力性药物的作用方面并不重要。

Jeacocke S A, England P J

Biochem J. 1980 Jun 15;188(3):763-8. doi: 10.1042/bj1880763.

A method was developed for the isolation of essentially pure myosin light chains from perfused rat heart. The phosphorylation of the P-light chains was estimated by hydrolysis and measurement of phosphate released, by electrophoresis in 8 M-urea and by 32P incorporation in perfusion with [32P]Pi. 2. In control perfusions there was 0.5-0.6 mol of phosphate/mol of P-light chain. This was not changed by perfusion with 5 microM-adrenaline for 10-40s. Perfusion for 1 min with medium containing 7.5 mM-CaCl2, or for 30s with medium containing 118 mM-KCl, also did not change the phosphorylation of P-light chains. 3. It is concluded that phosphorylation of P-light chains is not important in mediating the action of inotropic agents in the heart.

开发了一种从灌注大鼠心脏中分离基本纯的肌球蛋白轻链的方法。通过水解和测量释放的磷酸盐、在8M尿素中进行电泳以及在[32P]Pi灌注中进行32P掺入来估计P轻链的磷酸化。2. 在对照灌注中，每摩尔P轻链有0.5 - 0.6摩尔磷酸盐。用5微摩尔肾上腺素灌注10 - 40秒不会改变这一情况。用含7.5毫摩尔氯化钙的培养基灌注1分钟，或用含118毫摩尔氯化钾的培养基灌注30秒，也不会改变P轻链的磷酸化。3. 得出的结论是，P轻链的磷酸化在介导心脏中变力性药物的作用方面并不重要。