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[微秒延迟小球藻荧光]

[Microsecond delayed Chlorella fluorescence].

作者信息

Latsko G, Maroti Z, Ringler A, Shalan L

出版信息

Biofizika. 1980 May-Jun;25(3):531-6.

PMID:7397267
Abstract

The kinetics of delayed fluorescence between 40 microseconds and 500 microseconds at room temperature, excited by nanosecond light pulses of a nitrogen laser was analysed in terms of exponential components due to the different ways of reduction of the oxidized reaction centre pigment of the second photochemical system of photosynthesis. The details of the laser phosphoroscope of 20 microseconds resolution time are given. The delayed fluorescence of intact samples has 30 microseconds and 150 microseconds components; treatment with DCMU causes quenching, the kinetics remains unchanged; hydroxylamine causes enhancement at lower concentrations and quenching at higher ones, the component of 150 microseconds disappears. These changes corrborate the heterogeneous center model of the microsecond delayed fluorescence.

摘要

在室温下,利用氮激光的纳秒光脉冲激发,对40微秒至500微秒之间的延迟荧光动力学进行了分析,根据光合作用第二个光化学系统氧化反应中心色素还原的不同方式,将其分解为指数成分。给出了分辨率时间为20微秒的激光荧光镜的详细信息。完整样品的延迟荧光具有30微秒和150微秒的成分;用二氯苯基二甲基脲(DCMU)处理会导致猝灭,动力学保持不变;羟胺在较低浓度下会增强荧光,在较高浓度下会导致猝灭,150微秒的成分消失。这些变化证实了微秒延迟荧光的异质中心模型。

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