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[光系统II的快速和延迟荧光偏振]

[Polarization of prompt and delayed fluorescence of photosystem II].

作者信息

Guliaev B A, Teten'kin V L, Matorin D N

出版信息

Biofizika. 1982 Jan-Feb;27(1):42-7.

PMID:7066401
Abstract

Prompt and delayed fluorescence polarization of the oriented samples of synchronous. Chlorella vulgaris culture is investigated. Two main components with different polarization are revealed in the prompt and delayed fluorescence, however its ratio depends on the emission nature. The delayed fluorescence polarization ratio indicates that the reaction center pigment of PS-II dipole transition moment is in the photosynthetic membrane plane. The heterogeneity of variable and delayed PS-II fluorescence emission can be explained by the existence of two components: one of them is related to pigment-protein complex of PS-II (PPC-II) structurally and migrationally connected with LH-complex (alpha-component), but the other one--to PPC-II setting apart from the main LH-complex bulk (beta-component).

摘要

研究了同步培养的普通小球藻定向样品的瞬时光致荧光偏振和延迟光致荧光偏振。在瞬时光致荧光和延迟光致荧光中发现了具有不同偏振的两个主要成分,但其比例取决于发射性质。延迟光致荧光偏振比表明PS-II偶极跃迁矩的反应中心色素位于光合膜平面内。可变和延迟的PS-II荧光发射的异质性可以用两个成分的存在来解释:其中一个在结构上和迁移上与LH复合物相关,属于PS-II色素-蛋白复合物(PPC-II)(α成分),而另一个则是与主要的LH复合物主体分离的PPC-II(β成分)。

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